Protein diffusion from microwells with contrasting hydrogel domains
نویسندگان
چکیده
منابع مشابه
Multilayered heparin hydrogel microwells for cultivation of primary hepatocytes.
The biomaterial scaffolds for regenerative medicine need to be rationally designed to achieve the desired cell fate and function. This paper describes the development of hydrogel microstructures for cultivation of primary hepatocytes. Four different micropatterned surfaces are tested: 1) poly(ethyelene glycol) (PEG) microwells patterned on glass, 2) heparin hydrogel microwells patterned on glas...
متن کاملNanofibrillar hydrogel scaffolds from recombinant protein‐based polymers with integrin‐ and proteoglycan‐binding domains
This study describes the design, production, and testing of functionalized variants of a recombinant protein-based polymer that forms nanofibrillar hydrogels with self-healing properties. With a view to bone tissue engineering applications, we equipped these variants with N-terminal extensions containing either (1) integrin-binding (RGD) or (2) less commonly studied proteoglycan-binding (KRSR) ...
متن کاملDiscovering Domains Mediating Protein Interactions
Background: Protein-protein interactions do not provide any direct information regarding the domains within the proteins that mediate the interactions. The majority of proteins are multi domain proteins and the interaction between them is often defined by the pairs of their domains. Most of the former studies focus only on interacting domain pairs. However they do not consider the in...
متن کاملSynthesis of magnetic hydrogel microparticles for bioassays and tweezer manipulation in microwells
We report new methods for the synthesis and efficient manipulation of magnetic hydrogel microparticles. Through the development of a high-pH rinsing scheme, we achieve a simple and flexible synthesis strategy for the generation of geometrically and chemically complex magnetic microgels, eliminating the need for perfusion streams and other features that limit production rates and particle comple...
متن کامل7A Diffusion in domains with switching boundaries
In the analysis of narrow escape problems in Sect. 7.2, we assumed that the holes in the boundary of the domain were always open. However, if we view each pore as a stochastically gated ion channel, for example, then one has the problem of analyzing the diffusion equation in a domain with a randomly switching boundary (or partial boundary). Such a scenario also arises in the case of membrane di...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: APL Bioengineering
سال: 2019
ISSN: 2473-2877
DOI: 10.1063/1.5078650